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Showing: 1441 - 1460 of 4770 results

  1. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

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  2. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  3. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Use
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175591-plasmid-map-sequence-id-348365

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  4. pMesh1

    Plasmid
    #175594
    Purpose
    Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175594-plasmid-map-sequence-id-348364

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  8. pCarCBA

    Plasmid
    #117754
    Purpose
    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117754-plasmid-map-sequence-id-229023

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  9. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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  10. pUB_SM-FLuc0_EMCV-Ren

    Plasmid
    #210590
    Purpose
    Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase
    Depositor
    Olivia Rissland
    Article
    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi
    Inserts
    Spaghetti monster (FLAG)-nonoptimal FLuc
    Optimal Renilla luciferase
    Use
    Luciferase
    Tags
    Spaghetti monster (FLAG)
    Expression
    Mammalian
    Mutation
    Promoter
    EMCV IRES and UBC human
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210590-plasmid-map-sequence-id-416804

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  13. pENTR-ABCC1_STOP

    Plasmid
    #221423
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Insert
    ABCC1 (ABCC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221423-plasmid-map-sequence-id-437189

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  15. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169390-plasmid-map-sequence-id-336867

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  16. pHH0103 FNBP3 WW domain #1

    Plasmid
    #104233
    Purpose
    Bacterial expression of WW domain #1 from FNBP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP3 WW domain #1 (PRPF40A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104233-plasmid-map-sequence-id-204803

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  17. GSG2

    Plasmid
    #38915
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GSG2 (HASPIN Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. ATAD2B

    Plasmid
    #39046
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ATAD2B (ATAD2B Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. SETD2

    Plasmid
    #25348
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD2 (SETD2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. TAF1L

    Plasmid
    #39014
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1L (TAF1L Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1441 - 1460 of 4770 results