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Addgene
Showing: 1441 - 1460 of 4623 results
  1. pHH0103 ARHGAP9 WW domain

    Plasmid
    #104286
    Purpose
    Bacterial expression of WW domain from ARHGAP9
    Insert
    ARHGAP9 WW domain (ARHGAP9 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
  2. pHH0103 IQGAP3 WW domain

    Plasmid
    #104289
    Purpose
    Bacterial expression of WW domain from IQGAP3
    Insert
    IQGAP3 WW domain (IQGAP3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
  3. pHH0103 CEP164 WW domain

    Plasmid
    #104300
    Purpose
    Bacterial expression of WW domain from CEP164
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
  4. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Availability
    Academic Institutions and Nonprofits only
  5. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Availability
    Academic Institutions and Nonprofits only
  6. pET3-[Δ1-32]ARL10-His6(CO)

    Plasmid
    #201876
    Purpose
    Used for bacterial expression of human protein ARL10
    Depositor
    Insert
    ARL10 (ARL10 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Deletion of residues 1-32 from N-terminus and cod…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pCCL-c-MNDU3-CD3D-274+5G>A

    Plasmid
    #198023
    Purpose
    LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
    Depositor
    Insert
    CD3D c.274+5G>A
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211342
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Insert
    m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  9. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211343
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Insert
    m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  10. m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211344
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Insert
    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  11. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
  12. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Insert
    tet(A)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Availability
    Academic Institutions and Nonprofits only
  13. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Availability
    Academic Institutions and Nonprofits only
Showing: 1441 - 1460 of 4623 results