Addgene

We narrowed to 3,954 results for: chm

Showing: 1441 - 1460 of 3954 results

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFUS_A

Plasmid

#31028

Depositor

Daniel Voytas, Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-FLAG-Core-R (pMO29)

Plasmid

#24480

Depositor

Melanie Ott

Article

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Use

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pFUS_B2

Plasmid

#31019

Depositor

Daniel Voytas, Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P31

Plasmid

#120042

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-12

Plasmid

#119991

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Expression

Mutation

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTEF6-7

Plasmid

#124583

Purpose

Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymVenus-URA3

Plasmid

#168058

Purpose

Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_449

Plasmid

#179093

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpG

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-EF1a-Puro

Plasmid

#127170

Purpose

PCR template for EF1a-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_275

Plasmid

#179091

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-NG

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-15

Plasmid

#119994

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Expression

Mutation

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-CMV-Puro

Plasmid

#127169

Purpose

PCR template for CMV-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-HA-mAVO3 (pRL62-3)

Plasmid

#39210

Depositor

Michael Hall

Article

Jacinto et al Nat Cell Biol. 2004 Nov;6(11):1122-8. Ep

Insert

mAVO3 (Rictor Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G5S and I15V

Promoter

CMV

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pET3a-hUb

Plasmid

#61937

Purpose

Expression of hUb in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

human ubiquitin (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pEF-BE4Gam

Plasmid

#138269

Purpose

Expression of BE4am-Cas9 Base-editor from an EF1a promoter.

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BE4Gam

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDM_noro_A2_lacZ

Plasmid

#118820

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAM5057

Plasmid

#120085

Purpose

Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

Depositor

Susan Golden

Article

Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

Insert

RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-22

Plasmid

#120001

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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