1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pOCC230

   Plasmid

   #118871

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and mCitrine, cl…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOCC69

   Plasmid

   #118874

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and mGFP, cleava…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOCC110

   Plasmid

   #118905

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and LPETGg

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOCC219

   Plasmid

   #118908

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
   

   Use

   Tags

   YBBR tag, HIS10, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BB1_12_pDAS1

   Plasmid

   #98515

   Purpose

   BB1 with insert: promoter PDAS1

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   DAS1 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PFA0445w-bio-His

   Plasmid

   #50811

   Purpose

   Expresses enzymatically monobiotinylated full length PFA0445w

   Depositor

   Gavin Wright

   Article

   Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

   Insert

   PFA0445w
   

   Use

   Tags

   ratCD4d3+4,biotinylation sequence, 6xHis

   Expression

   Mammalian

   Mutation

   all potential N-linked glycosylation sites (N-X-S…

   Promoter

   CMV

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PF10_0166-bio-His

   Plasmid

   #50801

   Purpose

   Expresses enzymatically monobiotinylated full length PF10_0166

   Depositor

   Gavin Wright

   Article

   Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

   Insert

   PF10_0166
   

   Use

   Tags

   ratCD4d3+4,biotinylation sequence, 6xHis

   Expression

   Mammalian

   Mutation

   all potential N-linked glycosylation sites (N-X-S…

   Promoter

   CMV

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJT3

   Plasmid

   #98304

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBGO

   Plasmid

   #62671

   Purpose

   Donor vector for mFTH1 knock-in via landing pad

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   FTH1
   

   Use

   Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-Cx43

    Plasmid

    #65439

    Purpose

    Cloning, T7 RNA expression

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    T7 in vitro rna production

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL80037

    Plasmid

    #68260

    Purpose

    Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Coding sequence, neomycin phophotransferase II (Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL11061

    Plasmid

    #68255

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_AtU6-26 + sgRNA_BolC.GA4a-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICSL11058

    Plasmid

    #68254

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_TaU6 + sgRNA_HvPM19_3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFlp-Gm

    Plasmid

    #222335

    Purpose

    flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Use

    Tags

    9xMyc

    Expression

    Yeast

    Mutation

    Promoter

    pADH1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

    Plasmid

    #207024

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A Y387A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

    Plasmid

    #207023

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

    Plasmid

    #207022

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Y387A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

    Plasmid

    #207021

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge