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Plasmid#218827PurposeYeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFPDepositorInsertpT231 tau scFv
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144
Plasmid#69296PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGF012
Plasmid#48849PurposeProvides a sulfadiazine resistance cassette as GreenGate module.DepositorInsertpMAS::SulfadiazineR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition sites in SulfR removed …PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146
Plasmid#69298PurposeGolden Gate recipient and Gateway entry vector; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneUseTagsFokI nuclease (ELD)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA1
Plasmid#199727PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorInsertOsU3-sgRNA1 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA2
Plasmid#199728PurposeGolden Gate entry vector; 2nd gRNA under OsU3 promoterDepositorInsertOsU3-sgRNA2 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145
Plasmid#69297PurposeGolden Gate recipient and Gateway entry vector; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD151 ZF37i
Plasmid#138778PurposeConstitutive expression of ZF37i under the CMV promoter.DepositorInsertZF37i
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL11015
Plasmid#117499PurposeFAST-Red selectable marker Golden Gate Level 1 Position 1DepositorInsertBpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
UseTagsRFPExpressionPlantMutationPromoterAtOLE1Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyTagsExpressionMutationG65S, L68V, A72S, Y203T, L231HPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCJJ345
Plasmid#117504PurposeYAO:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtYAOAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA3
Plasmid#199729PurposeGolden Gate entry vector; 3rd gRNA under TaU3 promoterDepositorInsertTaU3-sgRNA3 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA4
Plasmid#199730PurposeGolden Gate entry vector; 4th gRNA under ZmU6-C1 promoterDepositorInsertZmU6C1-sgRNA4 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA5
Plasmid#199731PurposeGolden Gate entry vector; 5th gRNA under ZmU6-C3 promoterDepositorInsertZmU6C3-sgRNA5 scaffold
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only