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Plasmid#195230PurposeRetroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.DepositorInsertPKCB1 (PRKCB Human)
UseRetroviralTagsExpressionMutationK371RPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-PKCBII
Plasmid#195232PurposeMammalian expression vector containing GFP tagged PKCBII.DepositorInsertPKCB2 (PRKCB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17WHC
Plasmid#118839PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17WHC (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationWHC: L46W, V53H, E57CPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17FNV
Plasmid#118838PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17FNV (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationFNV: L46F, V53N, E57VPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR06
Plasmid#69152Purposeread-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr IIDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0 and codon-optimzed index…Promoterrps-27Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T-1 GST-Ago2 S387A
Plasmid#60656PurposeExpresses GST-Ago2 S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsGSTExpressionBacterialMutationS387APromotertacAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
Plasmid#128278PurposeExpresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell cultureDepositorInserttwinstar (tsr) (tsr Fly)
UseTags6X Histidine tagExpressionBacterialMutationMet1 changed to Pro1; Cys77 silent basepair mutat…PromoterT7Available sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)
Plasmid#194234PurposeLentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2
Plasmid#59904PurposeExpresses 6xHis-Ago2 (N-termi tag) in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387E
Plasmid#59906PurposeExpresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationS387E, phospho-dominantPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro
Plasmid#201677PurposeEBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassetteDepositorInsertMIR302-3g-EEA-2g-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExp-His-zBasic-RBD-Avi
Plasmid#195000PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsAvi-tag and His8-ZbasicExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
S. rosetta Col- cDNA amplified library
Pooled Library#62843Purposeamplified library of Salpingoeca rosettaDepositorExpressionBacterialSpeciesSalpingoeca rosettaAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387E
Plasmid#60653PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsCMV-FLAG-Ago2 (N-term tag)ExpressionMammalianMutationS387E mutantPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387A
Plasmid#60652PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsCMV-FLAG-Ago2 (N-term tag)ExpressionMutationS387A mutantPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387A
Plasmid#59905PurposeExpresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationS387A, phospho-null alaninePromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 5A
Plasmid#60651PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated five serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-ChR2-EGFP
Plasmid#114216PurposeExpresses channelrhodopsin-EGFP fusion in CamKIIa+ neuronsDepositorInsertChannelrhodopsin 2
UseAAV and Mouse TargetingTagsEGFPExpressionMammalianMutationPromoterCamKIIa 1.3 kbAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCer-TG2-YFP
Plasmid#85471PurposeTG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRETDepositorInserthTG2 (TGM2 Human)
UseTagsEYFP and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only