Addgene

We narrowed to 4,736 results for: KRAS

Showing: 1421 - 1440 of 4736 results

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AP56-6

Plasmid

#65593

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-5

Plasmid

#65591

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-8

Plasmid

#65596

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-7

Plasmid

#65594

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP54-3

Plasmid

#65597

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
mCerulean3-Keratin-17

Plasmid

#55424

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Keratin (KRT18 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Lasp1-N-10

Plasmid

#55426

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Lasp1 (LASP1 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

A209V

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-CDC42-C-10

Plasmid

#55406

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

CDC42 (CDC42 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
BRCA1-(936-1113)

Plasmid

#35075

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Use

Tags

His

Expression

Mutation

Contains aa 936-1113

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(1113-1316)

Plasmid

#35077

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Use

Tags

His

Expression

Mutation

Contains aa 1113-1316

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(894-1057)

Plasmid

#35074

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Use

Tags

His

Expression

Mutation

Contains aa 894-1057

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
CROPseq-Guide-Puro

Plasmid

#86708

Purpose

Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

Depositor

Christoph Bock

Article

Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T-hLIG3

Plasmid

#81055

Purpose

bacterial expression of human LIG3

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

DNA ligase 3 (LIG3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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WN10150

Plasmid

#80443

Purpose

Expresses dead (908A) AsCpf1

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

nuclease dead (908A) AsCpf1

Use

Tags

NLS-3xHA

Expression

Mammalian

Mutation

nuclease dead (D908A)

Promoter

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Mutation

Promoter

T7-lac

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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DRH335: FCK-Optopatch1

Plasmid

#51695

Purpose

Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1

Plasmid

#79503

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-MAPTau-C-10

Plasmid

#55431

Purpose

Localization: Microtubules, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MAPTau (MAPT Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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