-
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP
Plasmid#18696DepositorInsertmonomeric EGFP
UseTagsExpressionMammalianMutationA206K mutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 ONE-GO
Plasmid#189737PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry
Plasmid#207668PurposeTo express fluorescently labeled MS2 coat binding protein (MCP)DepositorInsertMCP-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-SopF
Plasmid#135174PurposeExpresses an mCherry-SopF fusion in mammalian cellsDepositorInsertSopF (STM1239 Salmonella enterica serovar Typhimurium)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV uN2CpolyGly op GFP (NIID)
Plasmid#224356PurposeExpress GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)DepositorInsertuN2C with a GGC repeat expansion (100x)
UseAAVTagseGFPExpressionMammalianMutationCodon-optimized for expression in human, so no pu…PromoterCMV + chimeric intron (CAG)Available sinceFeb. 5, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV pCAG-FLEX-EGFP-WPRE (AAV Retrograde)
Viral Prep#51502-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hIFITM3
Plasmid#58397PurposeExpressed human IFITM3 with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-4mtGCaMP6f-DIO
Plasmid#179529PurposeCre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoterDepositorInsert4mtGCaMP6f
UseAAVTags4x Cox8 targeting sequenceExpressionMutationPromoterEf1aAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNA repair library with 1,573 sgRNAs
Pooled Library#177663PurposeCRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processesDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)
Viral Prep#55645-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-MMLV_RT (lack H)
Plasmid#165556PurposeSingle 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.DepositorInsertMoloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAXY0002
Plasmid#179835Purposebinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorTypeEmpty backboneUseBinary vector for plant transformationTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
UseTagsExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag
Plasmid#167274PurposeOverexpression of CLIP-tag in E. coliDepositorInsertCLIP-tag
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pARC8-LambdaRedBeta-EcSSB
Plasmid#162572PurposeArabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.DepositorInsertsLambda Red-Beta
E. coli SSB
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX_sgRNA_entry (CJT32)
Plasmid#181782PurposeExpresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only