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Plasmid#194220PurposeBackbone for constructing single casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)
Plasmid#194221PurposeBackbone for constructing multi casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK Promoter (MTK 2) (pAN2822)
Plasmid#194224PurposeMammalian Toolkit part 2 encoding the PGK promoterDepositorInsertPGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a Promoter (MTK 2) (pAN2824)
Plasmid#194225PurposeMammalian Toolkit part 2 containing the EF1a promoterDepositorInsertEF1a Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
VPS13D(∆ATG2-C/PH)^EGFP
Plasmid#194004PurposeDeletion of DHPH domain of VPS13DDepositorInsertCodon optimized VPS13D (VPS13D Human)
UseTagsEGFPExpressionMammalianMutationearly stop codon before DHPHPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK234_058
Plasmid#123984PurposeEncodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsg Safetargeting Chr11
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_038
Plasmid#123917PurposeEncodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA-pSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_043
Plasmid#123913PurposeEncodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA-pSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP89b CvTA V124N
Plasmid#141207PurposeChromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
UseTagsExpressionBacterialMutationV124NPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_015
Plasmid#123991PurposeEncodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthROSA26 sg4
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8_005
Plasmid#123885PurposeEncodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK systemDepositorInsertF1-KanR-ColE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_021
Plasmid#123936PurposeEncodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK systemDepositorInsertTUL3R4 spacer
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_047
Plasmid#124055PurposeEncodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_048
Plasmid#124056PurposeEncodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_049
Plasmid#124057PurposeEncodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 4
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB340
Plasmid#124023PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713DepositorInsertLS-A4_EF1a-NLS::iRFP713-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB341
Plasmid#124024PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFPDepositorInsertLS-A4_EF1a-NLS::tagBFP-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only