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Plasmid#120045PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFPDepositorInsertTagBFP
UsePcr templateTagsTagBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet P2A Rac1-wt
Plasmid#125135PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.DepositorInsertmNeon/mScarletP2A-Rac1
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
II701: pMVP (L1-L4) cTnT promoter
Plasmid#121690PurposepMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertcTnT promoter (Cardiomyocyte specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IL-8 toehold switch sensor
Plasmid#111908PurposeToehold switch sensor to detect IL-8 mRNA with GFP outputDepositorInsertIL8 toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P17
Plasmid#120028PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCASP-HilA empty vector
Plasmid#153333PurposeTriggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressedDepositorTypeEmpty backboneUseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::tTA2-bGHpA
Plasmid#62447PurposeMXS_chaining vector with CMV::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with CMV promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts[mCherry]-1A(lacZ)
Plasmid#126980PurposeLevel 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationRepA A56V thermosensitive mutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z06
Plasmid#120049PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_MCS1
Plasmid#62395PurposeMXS_chaining vector with MCS1DepositorInsertEcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TGmiR_Luc-A
Plasmid#25765PurposeEntry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.DepositorInsertLuciferase miR-shRNA
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P9-PDGFR_EF1a_mPlum
Plasmid#186307PurposePlasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP9-PDGFR
mPlum
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP10-PDGFR_EF1a_mOrange
Plasmid#186308PurposePlasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line constructionDepositorInsertsAP10-PDGFR
mOrange
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P3-PDGFR_EF1a_iRFP670
Plasmid#186305PurposePlasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP3-PDGFR
iRFP670
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P32
Plasmid#120043PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only