Addgene

We narrowed to 3,401 results for: mak

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pCDF-SeAvs1

Plasmid

#188803

Purpose

Codon optimized SeAvs1 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs1

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMOTag-M9-4H

Plasmid

#106379

Purpose

Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS with M9 mutation

Use

Tags

Expression

Mutation

Promoter

Available since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4137387

Plasmid

#149544

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

wt;19-aa Ctrunc

Promoter

CMV

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits
pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D.R

Plasmid

#51510

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-DmrA

Plasmid

#61680

Purpose

GFP-labeled SAH scaffold with DmrA attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-DmrA

Use

Tags

Expression

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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human pirh2-(1-261)

Plasmid

#24878

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF-CcAvs4

Plasmid

#188808

Purpose

Codon optimized CcAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs4

Plasmid

#188806

Purpose

Codon optimized EcAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Luc-W

Plasmid

#196047

Purpose

lentiviral expression of the luciferase enzyme driven by cytomegalovirus virus promoter

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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MBP-TEV-VMA Model vector

Plasmid

#86590

Purpose

This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

VMA intein

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Original SapI site from pMal was mutated out

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pET29b_Qcat9

Plasmid

#45137

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT9

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat10

Plasmid

#45138

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT10

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Use

Tags

Expression

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat3

Plasmid

#45131

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT3

Use

Tags

5XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pZLSLD.R

Plasmid

#51511

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat5

Plasmid

#45133

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT5

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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