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Plasmid#89744PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLuc-OptoJNKi-dsm
Plasmid#89745PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertOptoJNKi dark-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CASK gRNA (BRDN0001149003)
Plasmid#77404Purpose3rd generation lentiviral gRNA plasmid targeting human CASKDepositorInsertCASK (CASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2556
Plasmid#144042PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertADNP2 (ADNP2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CASK gRNA (BRDN0001149003)
Plasmid#77404Purpose3rd generation lentiviral gRNA plasmid targeting human CASKDepositorInsertCASK (CASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA L514K L531E
Plasmid#40253DepositorInsertLOV-ipaA L514K L531E
UseTags6xHisExpressionBacterialMutationL514K; L531E; Jα helix region hybridized (AsLOV2 …PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
MDH1-shDusp5-2-H2Kb2
Plasmid#17853DepositorInsertshDusp5 (DUSP5 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-H2BmCherry-OptoJNKi
Plasmid#89741PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseOptogeneticsTagsHistone2B and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-dsm
Plasmid#89743PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertNLS-OptoJNKi dark-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-lsm
Plasmid#89742PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11DepositorInsertNLS-OptoJNKi lit-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationI539E mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLuc-Optop38i-lsm
Plasmid#89747PurposeExpression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13FDepositorInsertOptop38i lit-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationI539E mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLuc-OptoJNKi-lsm
Plasmid#89746PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11DepositorInsertOptoJNKi lit-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationI539E mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLuc-OptoJNKi
Plasmid#89744PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLuc-OptoJNKi-dsm
Plasmid#89745PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertOptoJNKi dark-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)
Plasmid#156404PurposeGateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseGateway cloningTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214T
Plasmid#51079Purposemammalian expression of ARAF S214TDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214TPromoterAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
R777-E009 Hs.ARAF
Plasmid#70293PurposeGateway ORF clone of human ARAF [NM_001256196.1] with stop codon (for native or N-terminal fusions)DepositorInsertARAF (ARAF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E010 Hs.ARAF-nostop
Plasmid#70294PurposeGateway ORF clone of human ARAF [NM_001256196.1] without stop codon (for C-terminal fusions)DepositorInsertARAF (ARAF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214T
Plasmid#51079Purposemammalian expression of ARAF S214TDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214TPromoterAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only