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Dcp1 H40A-EGFP
Plasmid#201421PurposeBacterial protein expression of Dcp1 H40ADepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH40APromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJL1_CSL3
Plasmid#200173PurposeAmino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vectorDepositorInsertCSL3
UseTags6x His tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term SMT3 NikJC199U
Plasmid#174362PurposeExpression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term SMT3 NikJC199U
Plasmid#174210PurposeExpression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF1 (G180A)
Plasmid#175415PurposeEncodes His-MBP-TEV-ybbR-G180A eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutationG180APromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF1 (WT)
Plasmid#175414PurposeEncodes His-MBP-TEV-ybbR-WT eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH858
Plasmid#172176PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH859
Plasmid#172177PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH768v2
Plasmid#172173PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH769v2
Plasmid#172174PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL2080
Plasmid#155160PurposeExpresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_L266APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2081
Plasmid#155161PurposeExpresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF(Y261A_L265A_L266A)PromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1957
Plasmid#155151PurposeExpresses Strep-MlaF-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationadded Streptavidin tag on MlaF N-terminusPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2074
Plasmid#155155PurposeExpresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaB_T52EPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2076
Plasmid#155156PurposeExpresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_K47APromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2077
Plasmid#155157PurposeExpresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_E170QPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2078
Plasmid#155158PurposeExpresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_Y261APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only
