Showing: 1381 - 1400 of 1585 results
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Plasmid#194050PurposeTransient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-VC155
Plasmid#194051PurposeTransient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailabilityAcademic Institutions and Nonprofits only -
4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO
Plasmid#68356Purpose-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
FlpO recombinase
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6AvailabilityAcademic Institutions and Nonprofits only -
pCAG-YTB
Plasmid#26721DepositorInsertUseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-FUCCI
Plasmid#118616PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailabilityAcademic Institutions and Nonprofits only -
RT3REVIN
Plasmid#111167PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsDsRed and mVenusExpressionMutationWTPromoterT3GAvailabilityAcademic Institutions and Nonprofits only -
pJK422
Plasmid#155373PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK423
Plasmid#155374PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK434
Plasmid#155378PurposePphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK503
Plasmid#155380PurposedCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#155384PurposedCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK520
Plasmid#155386PurposePphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA1 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-PTEN
Plasmid#168032PurposeExpresses eCFP-PTEN(M265-N329)-mVenus in bacterial cellsDepositorInserteCFP-PTEN(M265-N329)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VN154
Plasmid#194045PurposeTransient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VC155
Plasmid#194046PurposeTransient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1381 - 1400 of 1585 results