1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pOCC267

   Plasmid

   #118887

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease; HALO tag

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

   Plasmid

   #160507

   Purpose

   Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRW006-SM-destination-pro35S-Cas9

   Plasmid

   #104439

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   35S

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PliaG

   Plasmid

   #55174

   Purpose

   Constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   LiaG promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDH-hSyn-IntCCCre-T2A-CopGFP

   Plasmid

   #160503

   Purpose

   Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   IntCCCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   CopGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BB1_12_pGAP

   Plasmid

   #98498

   Purpose

   BB1 with insert: promoter PGAP

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   GAP promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-CMP-PE-V1

   Plasmid

   #202769

   Purpose

   Expresses CMP-PE-V1 in mammalian cells for prime editing

   Depositor

   Kyoungmi Kim

   Article

   Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

   Insert

   CMP-PE-V1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PSUC2-yEGFPCLN2PEST-pRS406

   Plasmid

   #64405

   Purpose

   SUC2 promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   SUC2 promoter (SUC2 Budding Yeast)
   

   Use

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Mutation

   Promoter

   SUC2

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ETV7_pet28a

   Plasmid

   #131656

   Purpose

   Bacterial expression of ETS gene ETV7

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV7 (ETV7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLBM0090

    Plasmid

    #172824

    Purpose

    Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    [35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Po_CyRPA-Cd4-bio-His

    Plasmid

    #126829

    Purpose

    Expression of recombinant P. ovale protein in mammalian cells

    Depositor

    Gavin Wright

    Article

    Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

    Insert

    CyRPA
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pm_CyRPA-Cd4-bio-His

    Plasmid

    #126817

    Purpose

    Expression of recombinant P. malariae protein in mammalian cells

    Depositor

    Gavin Wright

    Article

    Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

    Insert

    CyRPA (PmUG01_05040800 Plasmodium malariae)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF-XMAS-2xStop

    Plasmid

    #164412

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_Malat1_D

    Plasmid

    #72623

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLBT0160

    Plasmid

    #172812

    Purpose

    Level 1 module containing P19_P1 F

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    p35S-5UTMV-CDS1_P19-3U+TERNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICSL80036

    Plasmid

    #68259

    Purpose

    Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. J23101

    Plasmid

    #55176

    Purpose

    Very weak constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    J23101 promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge