-
Plasmid#162984PurposeTet-inducible shRNA targeting human METTL3 #2DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-Mettl3-dsRed-IRES-puro
Plasmid#61516PurposeConstitutive expression of murine wild-type Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGGAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362
Plasmid#24372DepositorInsertCLASP2 (340-1362) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-F2A-T2A-Venus
Plasmid#62621PurposeAdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tagDepositorInsertMCS with F2A and T2A
UseAdenoviralTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF708-pET28a-Hs-delta38-METTL20-NHis wt
Plasmid#85109Purposeexpression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta38) METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1084
Plasmid#24383DepositorInsertCLASP2 (340-1084) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Worm ABeta-attR5
Plasmid#160437PurposeEntry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Nematode, Human)
UseTagsCleavage signalExpressionBacterialMutationCodon-optimized for expression in C. elegansPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro METTL3
Plasmid#200724PurposeLentiviral expression of Flag-Tagged human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen
Plasmid#207123PurposeLentiviral vector to express human METTL3DepositorInserthMETTL3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24373DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1084 9xS/A
Plasmid#24384DepositorInsertCLASP2 (340-1084) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
Plasmid#182346PurposePacI-flanked CAG-LSL expression cassette with a FNF for positive selectionDepositorInserttdTomato
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR
Plasmid#207136PurposeDNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-inDepositorInsert5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterEc-TTL-P112 and Ec-TTL-P104Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BJ5183 cells
Bacterial Strain#16398DepositorBacterial ResistanceStreptomycinSpeciesEscherichia coliAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
UseTagsExpressionBacterialMutationPromoterEc-TTL-P112 and BBa_J23116Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.R363
Plasmid#138233PurposeExpresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-iGluSnFR.A184S (AAV1)
Viral Prep#106192-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.GFAP.SF-iGluSnFR.A184S (#106192). In addition to the viral particles, you will also receive purified pAAV.GFAP.SF-iGluSnFR.A184S plasmid DNA. GFAP-driven expression of SF-iGluSnFR.A184S. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsNoneAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only