Addgene

We narrowed to 52,531 results for: ran

Showing: 121 - 140 of 52531 results

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pET11a-hRanGAP1

Plasmid

#50941

Purpose

expression of full length untagged human RanGAP1 in E.coli

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanGTPase activating protein 1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_300

Plasmid

#124095

Purpose

orange calcium-modulated bioluminescent indicator, 300nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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mOrange2-ELP-1-25

Plasmid

#57954

Purpose

Localization: Golgi/ER, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

ELP (KDELR2 Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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KillerOrange /pQE-30

Plasmid

#74748

Purpose

KillerOrange gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

Insert

KillerOrange

Use

Tags

HisTag

Expression

Bacterial

Mutation

KillerRed with substitutions G3C Y66W D113S N145S…

Promoter

T5

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Roger Tsien, Erik Rodriguez

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange2-Lysosomes-20

Plasmid

#57968

Purpose

Localization: Lysosome Membrane, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Use

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pGEX4T2 RANK-L

Plasmid

#108571

Purpose

Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

Depositor

Jean-Claude Scimeca

Article

Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

Insert

RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

aa158-aa316

Promoter

Ptac (trp/lac)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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NPY-td-Orange2

Plasmid

#83497

Purpose

Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

Depositor

Sebastian Barg

Article

Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

Insert

NPY (NPY Human)

Use

Tags

tandem mOrange2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-PSmOrange

Plasmid

#31886

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Bacterial

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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hCD4-mOrange

Plasmid

#110192

Purpose

Encodes for human CD4 coding sequence tagged with the mOrange FP

Depositor

Sergi Padilla Parra

Article

Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

Insert

hCD4 (CD4 Human)

Use

Tags

mOrange

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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LeGO-mOrange2

Plasmid

#85212

Purpose

Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

mOrange2

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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amilCP_Orange chromoprotein

Plasmid

#117850

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Orange

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

64V,65G, BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Membrane mCherry

Plasmid

#210470

Purpose

Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

Depositor

Amro Hamdoun

Insert

LCK membrane mCherry (LCK Synthetic, Human, A.victoria)

Use

Synthetic Biology; Sea urchin, mammal, zebrafish

Tags

mCherry

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-RandE3

Plasmid

#79870

Purpose

Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 RanGAP

Plasmid

#13378

Depositor

Mary Dasso

Article

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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hCXCR4-LSSmOrange

Plasmid

#110197

Purpose

Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCXCR4 (CXCR4 Human)

Use

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Membrane mNeonGreen

Plasmid

#198057

Purpose

Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence

Depositor

Amro Hamdoun

Insert

LCK membrane mNeonGreen (LCK Human, A.victoria)

Use

Synthetic Biology; Sea urchin, mammal, zebrafish

Tags

mNeonGreen

Expression

Mutation

Tandem repeat of N-terminal LCK membrane targetin…

Promoter

sp6

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B-PSmOrange2

Plasmid

#34960

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Use

Tags

H2B

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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