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hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.
SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.