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Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
px459 VRER
Plasmid#101716PurposesgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)DepositorInsertSpCas9 VRER
UseTags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E and T1337R)PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-25:MAP1LC3B-mEGFP
Plasmid#101783PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3BDepositorInsertMAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4-UPRE-luc2P-Hygro
Plasmid#101788PurposeLuciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)DepositorInsert3xUPRE
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o2
Plasmid#101310PurposeContains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.DepositorInsertoriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE2-luc2P-Hygro
Plasmid#101790PurposeLuciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)DepositorInsert3xERSE-II
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNO4
Plasmid#101066PurposeBphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
5TEY (METTL14)
Plasmid#101891PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMETTL14 (METTL14 Human)
UseBaculovirus expressionTagsExpressionMutationPromoterpolyhedrinAvailable sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNO41
Plasmid#101067PurposeBphP1-PpsR2 chromophore plasmid; ho1DepositorInsertho1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB1532H-RFP
Plasmid#101855PurposeGolden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheTagsExpressionMutationPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB1532B-RFP
Plasmid#101854PurposeGolden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheTagsExpressionMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p16 nlsGPS1
Plasmid#101081PurposeGA biosensor expression plasmidDepositorInsertnlsGPS1
UseTagscMycExpressionPlantMutationPromoterp16 (AT3G60245)Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR FLIP43 GPS1
Plasmid#101084PurposeGA biosensor expression plasmidDepositorInsertGPS1
UseTags6HIS and cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB1532S-RFP
Plasmid#101856PurposeGolden-Gate compatible fungal transformation vector carrying Sulfonylurea Resistance (Chlorimuron Ethyl) with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p16 GPS1
Plasmid#101083PurposeGA biosensor expression plasmidDepositorInsertGPS1
UseTagscMycExpressionPlantMutationPromoterp16 (AT3G60245)Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
PPRE X3-TK-luc
Plasmid#1015PurposePPAR reporter. 3xDR1 sites upstream of a luciferase reporter.DepositorInsertPPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2005AvailabilityAcademic Institutions and Nonprofits only