Skip to main content
Addgene
Showing: 121 - 140 of 272 results

  12. Flag-2xAPEX2-C Cloning Vector

    Plasmid
    #136991
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136991-plasmid-map-sequence-id-271567

    Enlarge

  14. pET24a-VHH-tev-APEX2

    Plasmid
    #117751
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117751-plasmid-map-sequence-id-228349

    Enlarge

  15. AKT-APEX2-P2A-mKate2

    Plasmid
    #67654
    Purpose
    Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    AKT-PH-APEX2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67654-plasmid-map-sequence-id-116994

    Enlarge

  16. BTK-APEX2-P2A-mKate2

    Plasmid
    #67653
    Purpose
    Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    BTK-PH-APEX2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67653-plasmid-map-sequence-id-116993

    Enlarge

  17. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid
    #168207
    Purpose
    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection
    Depositor
    Markus Hafner
    Article
    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.
    Type
    Empty backbone
    Use
    Unspecified; Pdest gateway
    Tags
    V5 APEX2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168207-plasmid-map-sequence-id-331104

    Enlarge

  19. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid
    #67666
    Purpose
    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    mKate2-P2A-APEX2-ML1Nmut*2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67666-plasmid-map-sequence-id-117215

    Enlarge

Showing: 121 - 140 of 272 results