Addgene

We narrowed to 5,211 results for: pCas

Showing: 121 - 140 of 5211 results

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pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAT105

Plasmid

#180510

Purpose

Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
DpbCasX with R3 loop

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCas4_1_2_VA

Plasmid

#186438

Purpose

Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG:ECFP

Plasmid

#32597

Depositor

Anna-Katerina Hadjantonakis

Article

Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

Insert

ECFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Use

Tags

Expression

Mutation

Promoter

PccmK2

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAT207

Plasmid

#134884

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT203

Plasmid

#134880

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCasX1

Plasmid

#87685

Purpose

p15A plasmid expressing CasX locus from Deltaproteobacteria

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasX1

Use

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCA0.421

Plasmid

#203950

Purpose

Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAT527

Plasmid

#180514

Purpose

Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
PlmCasX with DpbCasX R1 loop-2A-mNeonGreen

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT218

Plasmid

#134886

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT024

Plasmid

#171636

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BFP
mTagBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT205

Plasmid

#134882

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT217

Plasmid

#134885

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_TWIST1

Plasmid

#215027

Purpose

Mammalian expression of human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAMBIA_ASX

Plasmid

#98888

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Article

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

CaMV35S (enhanced)

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT219

Plasmid

#134887

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_eGCaMP(2+)

Plasmid

#201148

Purpose

expresses eGCaMP2+ in mammalian cells

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP2+

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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