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Plasmid#205316PurposeAll-in-one AAV vector expressing ISAam1 TnpB and its reRNADepositorInserthumanized ISAam1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA
Plasmid#205320PurposeAll-in-one AAV vector expressing ISAba30 TnpB and its reRNADepositorInserthumanized ISAba30 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA
Plasmid#205319PurposeAll-in-one AAV vector expressing ISTfu1 TnpB and its reRNADepositorInserthumanized ISTfu1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-acceptor-2xBbsI
Plasmid#205633PurposeCloning and U6-drive expression of ISDra2 TnpB reRNAsDepositorInsertISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFPCPB1
Plasmid#23009DepositorInsertEGFP-CenpB
UseTagsEGFPExpressionMammalianMutationdeleted CenpB amino acids 169-606, T145SPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX234
Plasmid#23006DepositorInsertmRFP-CenpB
UseTagsmRFP1.0ExpressionMammalianMutationdeleted CenpB amino acids 169-606, T145SPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERB221
Plasmid#61502Purposeconst. CenpB-GFP-Halo + mCherry-DHFRDepositorInsertsCenpB-GFP-Halo
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPO640
Plasmid#195296PurposepTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).DepositorInsertminiMcCAST (Tn7575) with KanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO635
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO636
Plasmid#195299PurposepACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).DepositorInsertTnsABCQ operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO646
Plasmid#195307PurposepBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).DepositorInsertTnsD of McCAST
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO685
Plasmid#195300PurposepBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.DepositorInsertMcCAST Cascade and native array with PaqCI entry site
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO9Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPO717
Plasmid#195302PurposepBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.DepositorInsertMcCAST Cascade and lacZ spacer 5 in native array
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO812
Plasmid#195306PurposepBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.DepositorInsertMcCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-mmSCN9A
Plasmid#205636PurposereRNA guide targeting Mus Musculus SCN9ADepositorInsertreRNA-mmSCN9A (Scn9a Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPO794
Plasmid#195303PurposepBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.DepositorInsertMcCAST Cascade Δcas6 and ribozyme flanked entry site
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-hsRUNX
Plasmid#205639PurposereRNA guide targeting Homo sapiens RUNXDepositorInsertreRNA-hsRUNX (RUNX1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-mmPCSK9
Plasmid#205634PurposereRNA guide targeting Mus Musculus PCSK9DepositorInsertreRNA-mmPCSK9 (Pcsk9 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-hsEMX1
Plasmid#205637PurposereRNA guide targeting Homo sapiens EMX1DepositorInsertreRNA-hsEMX1 (EMX1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only