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Plasmid#68923PurposeNanoLuc expressed constiutively from PAM3 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT787
Plasmid#68922PurposeNanoLuc expressed constiutively from PAM3 and GH022 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT786
Plasmid#68921PurposeNanoLuc expressed constiutively from PAM3 and GH078 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT781
Plasmid#68918PurposeNanoLuc expressed constiutively from PAM2 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT776
Plasmid#68915PurposeNanoLuc expressed constiutively from PAM1 and RC500 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT775
Plasmid#68914PurposeNanoLuc expressed constiutively from PAM1 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT774
Plasmid#68913PurposeNanoLuc expressed constiutively from PAM1 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT772
Plasmid#68911PurposeNanoLuc expressed constiutively from PAM1 and GH078 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT753
Plasmid#68904PurposeNanoLuc expressed constiutively from PAM3, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT797
Plasmid#68930PurposeNanoLuc expressed constiutively from PAM4 and RC500 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM575
Plasmid#68881PurposeNanoLuc expressed constiutively from PcfiA, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM764
Plasmid#68901PurposeIPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYHY3
Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialMutationPromoterP1TDP-A21Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB701NL3F10H
Plasmid#141288PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYHY2
Plasmid#216108PurposeConstitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT0294 signal peptideExpressionBacterialMutationPromoterBfP1E6 promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB501NL3F10H
Plasmid#141286PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB601NL3F10H
Plasmid#141287PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB401NL3F10H
Plasmid#141285PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM763
Plasmid#68900PurposeIPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationG1104EPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only