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Plasmid#122729PurposeExpresses in mammalian cells (HEK293):glycosylated and secreted irisin-hisDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Bovine, Rat, Mouse, Human)
UseTagshis and signal sequence for secretionExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB013
Plasmid#119710PurposeTU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMTDepositorInsertNegative selection marker HSVtk (Pgpda:HSVtk:Ttub)
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3
Plasmid#124435PurposeChimeric ETS domain: H3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis TagExpressionBacterialMutationResidues 225 to 239 replaced with corresponding s…PromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3/S3
Plasmid#124627PurposeChimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 237 to 241 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-S3
Plasmid#124550PurposeChimeric ETS domain: S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 242 to 246 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS-H2
Plasmid#123358PurposeChimeric ETS domain: H2 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 207 to 215 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
J72008 bacterial strain with plasmid
Bacterial Strain#26187DepositorBacterial ResistanceAmpicillinAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMG10
Plasmid#31238DepositorTypeEmpty backboneUseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJC53.2/PRMT5
Plasmid#35692DepositorInsertProtein arginine methyltransferase 5
UseTagsExpressionMutationPromoterSp6 for sense, T7 for sense and antisenseAvailable sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D0000a
Plasmid#127230PurposeEmpty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A5801
Plasmid#127223Purpose35S::LuciferaseDepositorInsertLuc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A5803
Plasmid#127224PurposeCmYLCV::LuciferaseDepositorInsertLuc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH4-SYN-ChR2-tdimer2RFP
Plasmid#22772DepositorInsertChR2-tdimer2RFP
UseTagsChR2-tdimer2RFPExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbE0k-RFP
Plasmid#35365DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB0c-RFP
Plasmid#35366DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D4910
Plasmid#127220PurposeModule D with ZmUbi1::BBMDepositorInsertBBM
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB7k-GFP
Plasmid#35360DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbE7a-RFP
Plasmid#35313DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only