Ready-to-use AAV Retrograde particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-drive, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB hisG genes deleted.

Ready-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

To visualize membrane dynamics

mini-Tn7 cloning vector - empty backbone

sgRNA expression vector - promoterless backbone

sgRNA expression vector - pEM7 promoter

sgRNA expression vector - pBG35 promoter

sgRNA expression vector - pBG42 promoter

sgRNA expression vector - pBG28 promoter

mAPPLE labeled fluorescent actin

Express Orai1 in mammalian cells

Mammalian expression of GFP tagged MLC2. Wild-type

sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site