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Showing: 121 - 140 of 1098 results

  1. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  2. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  3. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80065
    Purpose
    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Zic2a enhancer driving c-fos promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80065-plasmid-map-sequence-id-152617

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  4. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  13. MCS-BioID2-HA pBabe-puro

    Plasmid
    #120308
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    -BioID2-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    120308-plasmid-map-sequence-id-239324

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  14. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Use
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  15. TBXTA-c043

    Plasmid
    #139766
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41- D225
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139766-plasmid-map-sequence-id-296581

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  16. TBXTA-c027

    Plasmid
    #139764
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139764-plasmid-map-sequence-id-296577

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  17. TBXTA-c028

    Plasmid
    #139765
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139765-plasmid-map-sequence-id-296580

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  18. TBXTA-c044

    Plasmid
    #139767
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41- D225
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139767-plasmid-map-sequence-id-296582

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Showing: 121 - 140 of 1098 results