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Showing: 121 - 140 of 1640 results

  1. A1-LCD-20G+20S+7F-7Y

    Plasmid
    #178688
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, seven Tyr …
    Promoter
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178688-plasmid-map-sequence-id-356407

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  2. A1-LCD-20G+20S-12F+12Y

    Plasmid
    #178689
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, twelve Phe…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178689-plasmid-map-sequence-id-356275

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  3. A1-LCD+23G-23S-12F+12Y

    Plasmid
    #178691
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, twel…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178691-plasmid-map-sequence-id-356195

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  4. A1-LCD+23G-23S+7F-7Y

    Plasmid
    #178690
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, seve…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178690-plasmid-map-sequence-id-356145

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  5. pIND4-RC-M210TAG-A7

    Plasmid
    #223015
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    A7 RS
    pylT tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    223015-plasmid-map-sequence-id-444740

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  6. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

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  7. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

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  8. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

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  9. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  10. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

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  12. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

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  19. pJK504

    Plasmid
    #71584
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-357 to tyrosine
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71584-plasmid-map-sequence-id-131755

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Showing: 121 - 140 of 1640 results