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Showing: 121 - 140 of 238 results

  1. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  2. pVH666

    Plasmid
    #169610
    Purpose
    Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169610-plasmid-map-sequence-id-366627

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  3. pVH664

    Plasmid
    #169608
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169608-plasmid-map-sequence-id-366623

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  4. pVH663

    Plasmid
    #169607
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169607-plasmid-map-sequence-id-365601

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  5. pWZ699

    Plasmid
    #208117
    Purpose
    Payload yeast assembly vector with TK-mActb on backbone
    Depositor
    Jef Boeke
    Article
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Type
    Empty backbone
    Use
    Synthetic Biology; Copy number inducible
    Tags
    Expression
    Bacterial, Mammalian, and Y…
    Mutation
    Promoter
    Human PGK1 for thymidine kinase
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208117-plasmid-map-sequence-id-409744

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  7. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

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  9. pMY.003

    Plasmid
    #228682
    Purpose
    pPGK1 Promoter for yeast expression with Nterminal tag
    Depositor
    Chen Davidovich
    Insert
    pPGK1
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    228682-plasmid-map-sequence-id-456174

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  14. pVG143

    Plasmid
    #227754
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Leu87Ile
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227754-plasmid-map-sequence-id-458037

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  15. pVG134

    Plasmid
    #227770
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84Gly mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227770-plasmid-map-sequence-id-458112

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  16. pVG178

    Plasmid
    #227761
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84KLys mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227761-plasmid-map-sequence-id-458111

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  17. pVG144

    Plasmid
    #227760
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val121Met mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227760-plasmid-map-sequence-id-458109

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  18. pVG150

    Plasmid
    #227759
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ser137Asn mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227759-plasmid-map-sequence-id-458108

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  19. pVG124

    Plasmid
    #227758
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Thr83Ser mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227758-plasmid-map-sequence-id-458107

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  20. pVG121

    Plasmid
    #227757
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Gly80Glu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227757-plasmid-map-sequence-id-458105

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Showing: 121 - 140 of 238 results