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We narrowed to 29,486 results for: Ide

Showing: 121 - 140 of 29486 results

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lentiguide-puro-wt-ltr

Plasmid

#182635

Purpose

Expresses guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro.3xBsmBI

Plasmid

#196709

Purpose

Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 EGFP-guide1

Plasmid

#118166

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 PSMB5 guide 1

Plasmid

#117073

Purpose

single guide RNA targeting PSMB5; guide 1

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

LMPX (PSMB5 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-PML peptide

Plasmid

#175246

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 ZFAND3-guide4

Plasmid

#125516

Purpose

CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 PSMB5 guide 2

Plasmid

#117074

Purpose

single guide RNA targeting PSMB5; guide 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

LMPX (PSMB5 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 EGFP-guide2

Plasmid

#118167

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Ideonella-bGSDM-WELQ

Plasmid

#222098

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Ideonella-bGSDM-WELQ ORF

Use

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-puro_Panc480-MT7

Plasmid

#200941

Purpose

Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

multiplexed sgRNA array

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, EF-1a

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide2 in pX458

Plasmid

#211536

Purpose

sgRNA-2 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide1 in pX458

Plasmid

#211535

Purpose

sgRNA-1 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 2

Plasmid

#193607

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 2 (INTS12 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 1

Plasmid

#193608

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 1 (GPAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPIGA guide 1

Plasmid

#193610

Purpose

PIGA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 1 (PIGA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPIGA guide 2

Plasmid

#193611

Purpose

PIGA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 2 (PIGA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPIGW guide 1

Plasmid

#193612

Purpose

PIGW knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGW guide 1 (PIGW Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgPIGW guide 2

Plasmid

#193613

Purpose

PIGW knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGW guide 2 (PIGW Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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