Addgene

We narrowed to 2,919 results for: GCG

Showing: 121 - 140 of 2919 results

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pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Expression

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Botch [N116/14R] (Recombinant Antibody)

Antibody

#191818-rAb

Purpose

Anti-Botch (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

March 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pAWp28-v1 scaffold

Plasmid

#157976

Purpose

pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAWp102-v2 scaffold

Plasmid

#157978

Purpose

pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Wolfe-Lawson ZFN modular assembly kit

Plasmid Kit

#1000000018

Purpose

Contains reagents for engineering zinc finger arrays by the modular assembly method.

Depositor

Nathan Lawson, Scot Wolfe

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNAS1B

Plasmid

#61968

Purpose

Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Serine 65 to Glycine (GGT), Serine 72 to Alanine …

Promoter

PBAD and PLtetO

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Use

Tags

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Promoter

Available since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pNAS1B

Plasmid

#61968

Purpose

Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Serine 65 to Glycine (GGT), Serine 72 to Alanine …

Promoter

PBAD and PLtetO

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-scramble

Plasmid

#118349

Purpose

This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

scrambled shRNA

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-shCtnnd2.2-GFP

Plasmid

#209097

Purpose

GFP expressing shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.2

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MYMH 47

Plasmid

#138038

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GCG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 29

Plasmid

#138029

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-5AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GCG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-ERR3 S57,81,219A

Plasmid

#37850

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

Estrogen-related receptor gamma (Esrrg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Serines 57, 81, and 219 to Alanine (AGC t…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only