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Showing: 121 - 140 of 501 results

  2. psLIB U6

    Plasmid
    #229956
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229956-plasmid-map-sequence-id-461573

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  3. psLIB CMV

    Plasmid
    #229953
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229953-plasmid-map-sequence-id-461575

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  4. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

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  5. psLIB TRE

    Plasmid
    #229955
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229955-plasmid-map-sequence-id-461253

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  6. pDONOR 6colors

    Plasmid
    #229974
    Purpose
    bigMamAct assembled plasmid for 6colors cellular labelling
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229974-plasmid-map-sequence-id-461259

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  11. pLP-PIGA (pLP140)

    Plasmid
    #168461
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Jef Boeke, Matthew Maurano
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168461-plasmid-map-sequence-id-362577

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  12. pPL001

    Plasmid
    #184750
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184750-plasmid-map-sequence-id-365374

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  13. pLP-PIGA2 (pLP300)

    Plasmid
    #168462
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168462-plasmid-map-sequence-id-362578

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  14. pLP-PIGA3 (pLP305)

    Plasmid
    #196992
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196992-plasmid-map-sequence-id-389805

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Showing: 121 - 140 of 501 results