Skip to main content
Addgene
Showing: 121 - 140 of 38499 results

  5. circRAJ31-Toehold_mutant

    Plasmid
    #69930
    Purpose
    Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.
    Depositor
    Alfonso Jaramillo
    Article
    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do
    Insert
    plTet-circRAJ31_toeholdmutant
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    4 bases mutated in toehold of riboregulator
    Promoter
    PltetO1-2
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    69930-plasmid-map-sequence-id-124217

    Enlarge

  7. VRQR variant

    Plasmid
    #138562
    Purpose
    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138562-plasmid-map-sequence-id-274786

    Enlarge

  10. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

    Enlarge

  12. cmv 16 E7 Q mutant

    Plasmid
    #13696
    Depositor
    Karl Munger
    Insert
    HPV 16 E7 Q mutant (E7 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    aa 30-37 changed from DSSEEEDE to QSSQQQQQ.
    Promoter
    Available since
    March 5, 2007
    Availability
    Academic Institutions and Nonprofits only

  15. fabp10a:H2B-mGreenLantern

    Plasmid
    #230045
    Purpose
    Expression of nuclear green fluorescent protein in zebrafish hepatocytes.
    Depositor
    Sumeet Pal Singh
    Insert
    mGreenLantern
    Use
    Zebrafish expression
    Tags
    H2B
    Expression
    Mutation
    Promoter
    fabp10a
    Available since
    Feb. 5, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230045-plasmid-map-sequence-id-460022

    Enlarge

  17. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid
    #191572
    Purpose
    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G and F486S (confers reduced binding affinity…
    Promoter
    CMV
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191572-plasmid-map-sequence-id-441975

    Enlarge

Showing: 121 - 140 of 38499 results