1. YFP-CD82

   Plasmid

   #1819

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   CD82 (CD82 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. lentiSAMv2 OPTN TSS-guide1

   Plasmid

   #118196

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiSAMv2 OPTN TSS-guide3

   Plasmid

   #118198

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiSAMv2 OPTN TSS-guide2

   Plasmid

   #118197

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiSAMv2 CAMK1D TSS-guide4

   Plasmid

   #118195

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiSAMv2 CAMK1D TSS-guide3

   Plasmid

   #118194

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiSAMv2 CAMK1D TSS-guide1

   Plasmid

   #118192

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiSAMv2 CAMK1D TSS-guide2

   Plasmid

   #118193

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Psin4-BCAT1

   Plasmid

   #196203

   Purpose

   overexpress BCAT1

   Depositor

   Jianjun Chen

   Insert

   BCAT1 (BCAT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NO

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJOE3075.3

   Plasmid

   #135084

   Purpose

   L-rhamnose-inducible expression vector

   Depositor

   Josef Altenbuchner

   Article

   Wegerer et al BMC Biotechnol. 2008 Jan 14;8:2. doi: 10

   Insert

   P-rhaP-BAD-malE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UAS-mCherry

    Plasmid

    #135457

    Purpose

    Mammalian mCherry reporter to read out FLARE and SPARK activation

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CA7

    Plasmid

    #39050

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CA7 (CA7 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

    Plasmid

    #135459

    Purpose

    Mammalian expression of SPARK protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-p2A-HA-βarrestin2-uTEV1Δ
    

    Use

    Lentiviral

    Tags

    eGFP, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

    Plasmid

    #135458

    Purpose

    Mammalian expression of SPARK TF component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
    

    Use

    Lentiviral

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Gal:mCherry-CRY2-uTEV1Δ(220-242)

    Plasmid

    #135452

    Purpose

    Yeast expression of uTEV1Δ fused to CRY2 and mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-CRY2-TEV1Δ(220-242)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Gal

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV:eGFP-CaM-uTEV1Δ(220-242)

    Plasmid

    #135461

    Purpose

    Mammalian expression of uFLARE protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-CaM-uTEV1Δ
    

    Use

    Tags

    eGFP, V5

    Expression

    Mammalian

    Mutation

    S219V mutation improves stability and S153N impro…

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Gal:mCherry-uTEV3

    Plasmid

    #135453

    Purpose

    Yeast expression of uTEV3 fused to mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-uTEv3
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    S219V mutation improves stability , I138T/S153N/T…

    Promoter

    Gal

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe puro MTH1

    Plasmid

    #21295

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    MTH1 (NUDT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pBabe hygro MTH1

    Plasmid

    #21296

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    MTH1 (NUDT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pLKO.1 shMTH1-1

    Plasmid

    #21297

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    shMTH1-1 (NUDT1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pLKO.1 shMTH1-2

    Plasmid

    #21298

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    shMTH1-2 (NUDT1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only