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Showing: 121 - 140 of 325 results

  2. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Article
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, Mustard Weed, S. aureus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Promoter
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

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  3. RPS1B-GFP (95)

    Plasmid
    #24039
    Depositor
    Michael Rout
    Insert
    RPS1B (RPS1B Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  4. R777-E165 Hs.PIK3CB

    Plasmid
    #70449
    Purpose
    Gateway ORF clone of human PIK3CB [NM_006219.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CB (PIK3CB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70449-plasmid-map-sequence-id-125898

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  5. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid
    #174893
    Purpose
    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
    Depositor
    Simon Elsaesser
    Insert
    mCherry and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174893-plasmid-map-sequence-id-344146

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  6. R777-E166 Hs.PIK3CB-nostop

    Plasmid
    #70450
    Purpose
    Gateway ORF clone of human PIK3CB [NM_006219.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CB (PIK3CB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70450-plasmid-map-sequence-id-358325

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  7. pET3-ARL8A-His6

    Plasmid
    #201872
    Purpose
    Used for bacterial expression of human protein ARL8A
    Depositor
    Richard Kahn
    Insert
    ARL8A (ARL8A Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201872-plasmid-map-sequence-id-403021

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  9. pCAG-Scarlet-Kif5a

    Plasmid
    #118745
    Purpose
    Expresses scarlet tagged Kif5a
    Depositor
    Beverly Koller
    Insert
    Kif5a cDNA (Kif5a Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118745-plasmid-map-sequence-id-229089

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  10. pScarlet-Kif5a

    Plasmid
    #118744
    Purpose
    Expresses scarlet tagged Kif5a
    Depositor
    Beverly Koller
    Insert
    Kif5a cDNA (Kif5a Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118744-plasmid-map-sequence-id-229075

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  11. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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  13. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  14. pFastBac Dual PNG172-STII PLU-HIS

    Plasmid
    #112992
    Purpose
    Baculovirus expression of Drosophila png172-Flag and plu-His
    Depositor
    Terry Orr-Weaver
    Inserts
    png172 (png Fly)
    plu (plu Fly)
    Use
    Tags
    Flag and His
    Expression
    Insect
    Mutation
    png172 mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112992-plasmid-map-sequence-id-217463

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  15. pFastBac Dual PNG-STII PLU-HIS

    Plasmid
    #112993
    Purpose
    Baculovirus expression of Drosophila png-Flag and plu-His
    Depositor
    Terry Orr-Weaver
    Inserts
    png (png Fly)
    plu (plu Fly)
    Use
    Tags
    Flag and His
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    112993-plasmid-map-sequence-id-217440

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  20. MK1283

    Plasmid
    #71428
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709
    Depositor
    Howard Salis
    Article
    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …
    Promoter
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71428-plasmid-map-sequence-id-440608

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Showing: 121 - 140 of 325 results