Skip to main content
Addgene
Showing: 121 - 140 of 107263 results

  2. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

    Enlarge

  18. pAF197

    Plasmid
    #135930
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    CTLA4 fused to IgG Fc and to mCherry
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135930-plasmid-map-sequence-id-270077

    Enlarge

  19. pAF160

    Plasmid
    #135925
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mAzurite
    Use
    Transposon
    Tags
    mAzurite
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135925-plasmid-map-sequence-id-270082

    Enlarge

  20. pAF164

    Plasmid
    #135928
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mOrange
    Use
    Transposon
    Tags
    mOrange
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135928-plasmid-map-sequence-id-270165

    Enlarge

Showing: 121 - 140 of 107263 results