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Plasmid#160647Purposemodule containing the human codon optimized Cas9 and the BASTA selection markerDepositorInsertBASTA / Cas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos, P35SAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 KIBRA WW domain #1
Plasmid#104229PurposeBacterial expression of WW domain #1 from KIBRADepositorInsertKIBRA WW domain #1 (WWOX Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 KIBRA WW domain #2
Plasmid#104230PurposeBacterial expression of WW domain #2 from KIBRADepositorInsertKIBRA WW domain #2 (WWC1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MYLK4
Plasmid#39175PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMYLK4 (MYLK4 Human)
UseBaculovirus expressionTagsHis TevExpressionMutationN371S, codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His
Plasmid#218198PurposeBacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOMDepositorInsertEndoCOM
UseTags3C-CBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 19-315PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3047-Left TALE-G1397-N-dead-mCherry
Plasmid#211339PurposeExpression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.3047-Left TALE-G1397-N-dead-mCherry
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3075-Left TALE-G1397-N-dead-mCherry
Plasmid#211340PurposeExpression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.3075-Left TALE-G1397-N-dead-mCherry
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.5147-Left TALE-G1397-N-dead-mCherry
Plasmid#211341PurposeExpression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.5147-Left TALE-G1397-N-dead-mCherry
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coUGP2
Plasmid#211531PurposeExpresses codon optimized UGP2DepositorInsertUGP2 (UGP2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARF6-His6(CO)
Plasmid#201887PurposeUsed for bacterial expression of human protein ARF6DepositorInsertARF6 (ARF6 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL1-His6 (CO)
Plasmid#201866PurposeUsed for bacterial expression of human protein ARL1DepositorInsertARL1 (ARL1 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hPhyB621(Y276H)-P2A-m1V
Plasmid#197377PurposeThe AAV vector for PhyB621(Y276H) and m1VDepositorInsertPhytochrome B Y276H(1-621 aa)
UseAAVTagsP2A-m1VExpressionMutationHuman codon optimized PhyB (1-621 aa), Y276H muta…PromoterCMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARF3-His6(CO)
Plasmid#201884PurposeUsed for bacterial expression of human protein ARF3DepositorInsertARF3 (ARF3 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 TCERG1L(ORF-2) WW domain #1
Plasmid#104294PurposeBacterial expression of WW domain #1 from TCERG1L(ORF-2)DepositorInsertTCERG1L(ORF-2) WW domain #1 (TCERG1L Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HYPB WW domain
Plasmid#104267PurposeBacterial expression of WW domain from HYPBDepositorInsertHYPB WW domain (SETD2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HYPC WW domain #2
Plasmid#104232PurposeBacterial expression of WW domain #2 from HYPCDepositorInsertHYPC WW domain #2 (PRPF40B Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIV298
Plasmid#204957PurposeAMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection markerDepositorInsertsMad7
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTRdeltaGFPmirGE-DroshaT1.5.5
Plasmid#61084PurposepENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.DepositorInsertdeltaGFP
UseRNAiTagsExpressionMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only