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Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGateway 3XFlag Prickle2 flag tagged
Plasmid#24645DepositorInsertPrickle 2 (PRICKLE2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceApril 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmCherry Tensin2
Plasmid#105300PurposeTo study localization and function of Tensin 2 with EGFP Tensin1DepositorInsertTensin2 (TNS2 Human)
UseTagsmCherryExpressionMammalianMutationlacks N-terminal C1 domainPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2
Plasmid#79255PurposeExpresses EGFP-tagged DmNot2 in S2 cellsDepositorInsertDmNot2 (Rga Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCCr4
Plasmid#79252PurposeExpresses EGFP-tagged DmCCr4 in S2 cellsDepositorInsertDmCaf40 (twin Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (P.1 lineage / Brazilian variant)
Plasmid#169847PurposeMammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tagDepositorInsertProtein S
UseTagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (B.1.1.7 lineage / UK variant)
Plasmid#169848PurposeMammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tagDepositorInsertProtein S
UseTagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)
Plasmid#194233PurposeMammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domainDepositorInsertCD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-wdb
Plasmid#195069PurposeExpresses FLAG-tagged Drosophila wdb proteinDepositorInsertwiderborst (wdb Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-EGFP-DmSquash_B
Plasmid#145926PurposeInsect Expression of DmSquashDepositorInsertDmSquash (squ Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.2-GFP
Plasmid#72908PurposeGFP-tagged mSin1 (isoform 2)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-W-FUGW
Plasmid#166751PurposeExpress mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-W (Bcl2l2 Mouse)
UseLentiviralTagsmCerulean3ExpressionMutationPromoterhuman ubiquitin CAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE GFP-Ago2 S387A
Plasmid#60654PurposeGFP-Ago2 S387A mutant lentiviral vectorDepositorInsertArgonaute 2 (AGO2 Human)
UseLentiviralTagsGFPExpressionMutationS387APromoterEF1aAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE GFP-Ago2 S387E
Plasmid#60655PurposeGFP-Ago2 S387E mutant lentiviral vectorDepositorInsertArgonaute 2 (AGO2 Human)
UseLentiviralTagsGFPExpressionMutationS387EPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Jam2-AP-His
Plasmid#71953PurposeExpresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertJam2 (Jam2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4-Fc-His
Plasmid#72084PurposeExpresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertLrrc4 (Lrrc4 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4-AP-His
Plasmid#71958PurposeExpresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertLrrc4 (Lrrc4 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2 GG
Plasmid#13384DepositorInsertSumo2 GG (SUMO2 Human)
UseTagsEGFP and GSTExpressionBacterialMutationMature SUMO2GG: diglycine at amino acid positions…PromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only