Addgene

We narrowed to 3,013 results for: actin

Showing: 1361 - 1380 of 3013 results

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pSNAPf-Nup43

Plasmid

#98276

Purpose

mammalian expression of SNAPf-Nup43

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup43 (NUP43 Human)

Use

Tags

SNAPf-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNup133-Ypet

Plasmid

#98283

Purpose

mammalian expression of Nup133-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup133 (NUP133 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTLg

Plasmid

#61195

Purpose

Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-eGFP

Use

Tags

eGFP

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-eGFP-F

Plasmid

#71545

Purpose

Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

Depositor

Pantelis Tsoulfas

Insert

eGFP-F

Use

AAV

Tags

contains 20- amino-acid farnesylation signal from…

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Use

Tags

Expression

Yeast

Mutation

Promoter

Actine

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTLr

Plasmid

#61194

Purpose

Fluorescent reporter for F-actin LifeAct-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTFr

Plasmid

#61192

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_STf

Plasmid

#182011

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lifeact_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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3xmTurquoise2_UtrCH

Plasmid

#112963

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

UtrCH

Use

Tags

3x mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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VinculinTS-I997A

Plasmid

#111828

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Use

Tags

Expression

Mammalian

Mutation

changed isoleucine 997 to alanine

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rab10

Plasmid

#31737

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab10 (Rab10 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
mCerulean3-Lifeact-7

Plasmid

#54721

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-Lifeact-GFP

Plasmid

#175437

Purpose

F-actin structures labelling

Depositor

Sebastián Dupraz, Frank Bradke

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lifeact-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

pTub-alpha1

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-UtrCH

Plasmid

#98824

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

UtrCH

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-Per

Plasmid

#33157

Depositor

Michael Rosbash

Article

Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

Insert

Per (per Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pDpsTE-Ypet

Plasmid

#98277

Purpose

mammalian expression of DpsTE-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Dps

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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ITPKA_3xmTurquoise

Plasmid

#112961

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

ITPKA

Use

Tags

3x mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase

Plasmid

#162556

Purpose

NheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Use

Tags

Expression

Yeast

Mutation

Promoter

Actine

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-iLifeact-NeonGreen

Plasmid

#175258

Purpose

F-actin structures labelling

Depositor

Sebastián Dupraz, Frank Bradke

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

iLifeact

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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