Addgene

We narrowed to 5,233 results for: pCas

Showing: 1341 - 1360 of 5233 results

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)

Plasmid

#203542

Purpose

Make FIP200 NTD:ATG13:ULK1 complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSH 1-178

Plasmid

#160050

Purpose

Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSH residues 1-178 (STAMBP Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-178

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-KATNB1

Plasmid

#160054

Purpose

Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNB1 (KATNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSHLP 16-218

Plasmid

#180609

Purpose

Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSHLP residues 16-218

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 16-218

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL2 residues 1-98

Plasmid

#180619

Purpose

Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL2 (KATNAL2 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-98

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-HAT

Plasmid

#202597

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-HAT

Plasmid

#202598

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

Plasmid

#197421

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

Plasmid

#197422

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

Plasmid

#197423

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

Plasmid

#197424

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

Plasmid

#197425

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m-ΔZF

Plasmid

#192276

Purpose

Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

Cas12m ΔZF

Use

CRISPR

Tags

Expression

Bacterial

Mutation

H459A, C552A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m-ΔZF

Plasmid

#192277

Purpose

pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m ΔZF

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D485A, H549A, C552A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-basic

Plasmid

#196882

Purpose

Used for preparation of double-floxed inverse ORF vectors

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Type

Empty backbone

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-1700029I15Rik-3×FLAG

Plasmid

#199689

Purpose

Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

Insert

1700029I15Rik (1700029I15Rik Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL(C145A)

Plasmid

#200121

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

C145A

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Tsks/1D4

Plasmid

#199623

Purpose

Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tsks (variant X6) (Tsks Mouse)

Use

Tags

1D4

Expression

Mutation

Promoter

CAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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