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Plasmid#101003PurposeConstruct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsExpressionMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCAS_gpyrG2
Plasmid#90277Purpose(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9DepositorInsertgRNA (pyrg2)
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90285PurposeBB2 with split pyrG marker for homologous integration with linker AB (negative control)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90286PurposeBB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90287PurposeBB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90288PurposeBB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-LGK
Plasmid#81165PurposepETconNK with levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
UseTagsc-mycExpressionBacterial and YeastMutationPromoterGAL1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB762
Plasmid#60150PurposeFirefly luciferase (Promega) driven by LEU1 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB767
Plasmid#60159PurposeFirefly luciferase (Promega) driven by SIC1 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationA4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q HDEL
Plasmid#32123DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A HDEL
Plasmid#32121DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-NLuc_SV40:PuroR
Plasmid#183045PurposeExpresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.DepositorInsertNanoLuciferase
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA iRFP713_zhang2.0
Plasmid#167916PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-MAPPER
Plasmid#117721PurposeMAPPER Selectively Labels ER-PM JunctionsDepositorInsertMAPPER
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-EXOB-11
Plasmid#197688PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEXOB enhancer-blocking insulator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
UseTagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro_GCaMP6s
Plasmid#164589PurposeStable integration and expression of GCaMP6s in mammalian cellsDepositorInsertGCaMP6s
UseLentiviralTags6xHis, T7 tag, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
WFS1-Flag-pcDNA3
Plasmid#13011DepositorInsertWolfram Syndrome 1 (WFS1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_12GLI-FLuc_R4-R3
Plasmid#113712PurposeSingle pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.DepositorInsert12GLI-FLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG
Plasmid#139961PurposeOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanicaDepositorInsertConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only