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Showing: 1341 - 1360 of 13305 results

  1. pAC1-pCR8-dCas9VP48

    Plasmid
    #48214
    Purpose
    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP48 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP48
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48214-plasmid-map-sequence-id-69493

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  2. TDK promoter dCas9

    Plasmid
    #113657
    Purpose
    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.
    Depositor
    Jeffrey Barrick
    Article
    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531
    Insert
    TDK gene promoter and dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TDK gene promoter
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    113657-plasmid-map-sequence-id-233276

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  4. pEN017-SpCas9-hiNLS-t-M4

    Plasmid
    #234455
    Purpose
    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG
    Depositor
    Ross Wilson
    Insert
    SpCas9 hiNLS t-M4
    Use
    CRISPR
    Tags
    His6, Nucleoplasmin, SV40, and c-Myc
    Expression
    Bacterial
    Mutation
    Cys at positions 80 and 574 have been mutated to …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234455-plasmid-map-sequence-id-464910

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  6. pdCas9-GFP-p65

    Plasmid
    #232630
    Purpose
    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-GFP-p65 (RELA Synthetic, Human)
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232630-plasmid-map-sequence-id-463624

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  7. pdCas9-tdTomato-VPR

    Plasmid
    #232632
    Purpose
    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato-VPR
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232632-plasmid-map-sequence-id-462149

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  10. pcDNA-Sga-Cas9

    Plasmid
    #227005
    Purpose
    Mammalian expression of Nuclease active S. gallolyticus Cas9
    Depositor
    Charles Gersbach
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227005-plasmid-map-sequence-id-449963

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  11. pcDNA-Sin-Cas9

    Plasmid
    #227006
    Purpose
    Mammalian expression of Nuclease active S. iniae Cas9
    Depositor
    Charles Gersbach
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227006-plasmid-map-sequence-id-449969

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  12. pcDNA-Spa-Cas9

    Plasmid
    #227007
    Purpose
    Mammalian expression of Nuclease active S. parasanguinis Cas9
    Depositor
    Charles Gersbach
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227007-plasmid-map-sequence-id-449962

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  13. pcDNA-Sub-Cas9

    Plasmid
    #227008
    Purpose
    Mammalian expression of Nuclease active S. uberis Cas9
    Depositor
    Charles Gersbach
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227008-plasmid-map-sequence-id-449970

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  15. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

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  16. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

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  17. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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  18. TRE3GV-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222503
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222503-plasmid-map-sequence-id-441222

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  19. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222505
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222505-plasmid-map-sequence-id-441223

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  20. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222506
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222506-plasmid-map-sequence-id-441224

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Showing: 1341 - 1360 of 13305 results