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Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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G15-CASE
Plasmid#168126PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at E244/N245 within GNA15PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EHC
Plasmid#112619Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2DepositorInsertsUseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K49R-STAT3
Plasmid#203721PurposeExpresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
UseTagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K49R substitutionsPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K685R-STAT3
Plasmid#203722PurposeExpresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
UseTagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K685R substitutionsPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)
Plasmid#135501PurposeMammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)DepositorInsertH2-Dd (H2-D1 Mouse)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationY84C/C121S/A139CPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TopBP1-mVen
Plasmid#211415Purposemammalian expression of human TopBP1 C-terminally fused to mVenusDepositorInsertTopBP1 (TOPBP1 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RecQL4-mVen
Plasmid#211403Purposemammalian expression of human RecQL4 C-terminally fused to mVenusDepositorInsertRecQL4 (RECQL4 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C16orf59-mVen
Plasmid#211425Purposemammalian expression of human C16orf59 C-terminally fused to mVenusDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
px330_SMAD exon 2 gRNA
Plasmid#68350Purposepx330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.DepositorInsertSMAD exon 2 gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330_P53 exon 8 gRNA
Plasmid#68349Purposepx330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.DepositorInsertP53 exon 8 gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330_P53 exon 7 gRNA
Plasmid#68351Purposepx330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.DepositorInsertP53 exon 7 gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330_PTEN exon 5 gRNA
Plasmid#68348Purposepx330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.DepositorInsertPTEN exon 5 gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330_SMAD exon 9 gRNA
Plasmid#68352Purposepx330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.DepositorInsertSMAD exon 9 gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdc45-mVen
Plasmid#211390Purposemammalian expression of human Cdc45 C-terminally fused to mVenusDepositorInsertCdc45 (CDC45 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm5-mVen
Plasmid#211394Purposemammalian expression of human Mcm5 C-terminally fused to mVenusDepositorInsertMcm5 (MCM5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53-mVen
Plasmid#211421Purposemammalian expression of human C17orf53 C-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm2-mVen
Plasmid#211398Purposemammalian expression of human Mcm2 C-terminally fused to mVenusDepositorInsertMcm2 (MCM2 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT0090
Plasmid#8714DepositorInsertyEVenus
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0103
Plasmid#8718DepositorInsertyEVenus
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only