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Plasmid#61848Purposefor making stable RPE cells expressing WT cdc20DepositorInsertcdc20 (CDC20 Human)
UseTagsFlag and VenusExpressionMammalianMutationwobbledPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGD007
Plasmid#48837PurposeProvides linker:nuclear localization signal as GreenGate C-terminal tag module.DepositorInsertlinker:nuclear localization signal
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Mouse Alpha2 delta-2 mutMIDAS HA pMT2
Plasmid#58733Purposeexpression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationMIDAS site mutations (D300VSGS to AVAGA)PromoterSV40Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCY 3050-07
Plasmid#36223DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-Venus-ZeocinExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-Kdrl-3p-FokI-KK
Plasmid#35685DepositorInsertKdrl-3p-FokI-KK
UseMrna productionTagsHAExpressionMutationPromoterAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-Kdrl-5p-FokI-EL
Plasmid#35684DepositorInsertKdrl-5p-FokI-EL
UseMrna productionTagsFLAG and FlagExpressionMutationPromoterAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-spo5DSR
Plasmid#49867Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pC0.425
Plasmid#203944PurposeUp Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.426
Plasmid#203945PurposeDown Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.391
Plasmid#203948PurposeLevel 0 part. Cas12a coding sequence from Francisella novicida in CDS1 positionDepositorInsertFnCas12a
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.420
Plasmid#203934PurposeUp Flank. Neutral site. Up flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.DepositorInsertaquI UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.385
Plasmid#203936PurposeUp Flank. Neutral site. Up flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.DepositorInsertdesB UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.386
Plasmid#203938PurposeUp Flank. Neutral site. Up flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.DepositorInsertglgA1 UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.387
Plasmid#203939PurposeDown Flank. Neutral site. Down flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.DepositorInsertglgA1 DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.388
Plasmid#203940PurposeUp Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only