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Plasmid#100565PurposehgRNA-G21 in a lentiviral backboneDepositorInserthgRNA-G21
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW091
Plasmid#113253PurposepUC19 backbone with S. pyogenes CRISPR arrayDepositorInsertS. pyogenes CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hgRNA-E21_pLKO-Hyg
Plasmid#100563PurposehgRNA-E21 in a lentiviral backboneDepositorInserthgRNA-E21
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
hgRNA-A21_pLKO-Puro
Plasmid#100570PurposehgRNA-A21 in a lentiviral backbone with puromycin resistanceDepositorInserthgRNA-A21
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ284
Plasmid#52225PurposeExpression of sgRNA precursor targeted against ade6+DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]
Plasmid#124069PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxTagsExpressionInsectMutationPromoterbhsp68Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterS.pombe rrk1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
FISSEQ-hgRNA-Design2
Plasmid#100572PurposeFISSEQ-hgRNA-Design2 in a lentiviral backboneDepositorInsertFISSEQ-hgRNA-Design2
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH235
Plasmid#121145PurposeCas9 expression controlled by the nmt41 promoterDepositorInserthumanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoternmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ285
Plasmid#52226PurposeExpression of sgRNA precursor targeted against ade6M210DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FISSEQ-hgRNA-Design1
Plasmid#100571PurposeFISSEQ-hgRNA-Design1 in a lentiviral backboneDepositorInsertFISSEQ-hgRNA-Design1
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRC055
Plasmid#225977PurposedRxCas13d expressed from pTet promoterDepositorInsertdRxCas13d
UseCRISPRTagsExpressionMutationPromoterpTetAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC64434
Plasmid#209518PurposeCloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNT-15
Plasmid#154932Purpose15 designed non-target sitesDepositorInsert15 designed non-target sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNT-12
Plasmid#154933Purpose12 designed non-target sitesDepositorInsert12 designed non-target sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNT-3
Plasmid#154934Purpose3 designed non-target sitesDepositorInsert3 designed non-target sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNT-1
Plasmid#154935Purpose1 designed non-target siteDepositorInsert1 designed non-target sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMZ286
Plasmid#52227PurposeExpression of sgRNA precursor targeted against ade6L469DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gDM1d
Plasmid#114387PurposepPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGGDepositorInsertgDM1d
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pX601 miniCMV-SaCas9-SpA-sgRNA scaffold
Plasmid#107055PurposeBackbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only