Addgene

We narrowed to 2,065 results for: Xpa

Showing: 1321 - 1340 of 2065 results

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pT2-7xTcf-NLS-ONL

Plasmid

#65713

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJT112_GalL_nCDA1-xBE3

Plasmid

#141318

Purpose

Expressing base editor nCDA1-xBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-xBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

Promoter

GalL

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMC234r-ccdB/TPK2

Plasmid

#176179

Purpose

yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

dropout cassette containing toxic genes ccdB and TPK2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AtS/MAR10 insulator sequence (GB3458)

Plasmid

#160638

Purpose

pEGB3a1 AtS/MAR10

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

AtS/MAR10 insulator sequence

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1759

Plasmid

#154315

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::TEV::AID*::3xFLAG for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71349

Purpose

Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)_H2B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pJW1806

Plasmid

#154319

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::3xMyc for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1805

Plasmid

#154318

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::3xMyc for CT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1348

Plasmid

#154311

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1822

Plasmid

#154322

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71345

Purpose

Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

hOTC_Cyan Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pHyg-AID(1-114)

Plasmid

#99513

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-L-V5-His

Plasmid

#222848

Purpose

E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

V5-His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3109 (pSpy1C-Plac(1)_ffluc)

Plasmid

#218514

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(1)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3111 (pSpy1C-Plac(3)_ffluc)

Plasmid

#218778

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(3)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger2

Plasmid

#217266

Purpose

Donor plasmid of Tigger2 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger2 transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-MarsTigger8

Plasmid

#217271

Purpose

Donor plasmid of MarsTigger8 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of MarsTigger8 transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger4

Plasmid

#217264

Purpose

Donor plasmid of Tigger4 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger4 transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger3

Plasmid

#217258

Purpose

Donor plasmid of Tigger3 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger3 transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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