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Showing: 1321 - 1340 of 1407 results

  1. pOmnibac_ZZ_linker_ybbR_Tau MTBD

    Plasmid
    #159719
    Purpose
    Expresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tag
    Depositor
    Ahmet Yildiz
    Article
    Ferro et al Science. 2022 Jan 21;375(6578):326-331.
    Insert
    Tau microtubule-binding domain only (amino acids 242-367) (MAPT Human)
    Use
    Tags
    ZZ affinity tag, ybbR tag
    Expression
    Insect
    Mutation
    Only amino acids 242-367
    Promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    159719-plasmid-map-sequence-id-313616

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  2. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Use
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  3. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid
    #169194
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells
    Depositor
    John Diffley
    Article
    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531
    Insert
    3xFlag-His6-PLpro (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    3xFlag-His6
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169194-plasmid-map-sequence-id-333980

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  7. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

    Plasmid
    #185447
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF1 (TERF1 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Use
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185447-plasmid-map-sequence-id-364982

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  11. pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

    Plasmid
    #187936
    Purpose
    Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327
    Depositor
    Sascha Martens
    Article
    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e
    Inserts
    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    Use
    Tags
    GST-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187936-plasmid-map-sequence-id-374446

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  12. pFBOH-LIC_SMU1:1-513

    Plasmid
    #210877
    Purpose
    Insect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    SMU1:M1-P513 (SMU1 Human)
    Use
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210877-plasmid-map-sequence-id-426048

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  16. pFBOH-MHL_SHKBP1:182-585

    Plasmid
    #210935
    Purpose
    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    SHKBP1:T182-L585 (SHKBP1 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    Q507L natural variant (VAR_036714)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210935-plasmid-map-sequence-id-426241

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Showing: 1321 - 1340 of 1407 results