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Plasmid#209722PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensorDepositorInsert(mito).iATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
MoClo Tool Kit
Plasmid Kit#1000000044PurposeEukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminatorsDepositorAvailable sinceApril 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLViP-LifeAct-iRFP670
Plasmid#229699PurposeLentiviral expression of LifeAct-iRFP670 in mammalian cellsDepositorInsertSynthetic construct LifeAct-mTurquoise gene
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Sec22b
Plasmid#101918PurposeExpresses Sec22b in mammalian cellsDepositorInsertSec22b (Sec22b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSWEET-bgaB
Plasmid#210272PurposeXylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.DepositorInsertbgaB
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-B2M-miniGag
Plasmid#228957PurposeminiEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
RelA cFlag pcDNA3
Plasmid#20012DepositorInsertRelA (Rela Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21 (ORF-EG)
Plasmid#46948PurposeInducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV PHP.eB)
Viral Prep#105530-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-7xHis-TEV-hexa
Plasmid#222868PurposeExpresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTags7xHis and MBPExpressionBacterialMutationC19V, L56V, C110V, C130S, S135G, S219V. Insert in…PromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-B2M-miniGag-Cas9
Plasmid#228958PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
BL21(DE3)-R3-pRARE2
Bacterial Strain#26242PurposePhage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.DepositorBacterial ResistanceChloramphenicolAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)
Viral Prep#162380-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV1)
Viral Prep#44362-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBB Flag GCN5
Plasmid#74784Purposeexpress of GCN5 in mammalian cellsDepositorInsertGCN5 (KAT2A Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCL/IFNB1-d2eGFP-3’UTR
Plasmid#180232PurposeReporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1DepositorInsertd2eGFP
UseLentiviralTagsExpressionMutationPromoter1 kb upstream of IFNB1 CDSAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only