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Plasmid#60857Purpose2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoterDepositorInsertmiR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterpTightAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mito-mStayGold_pTwistCMVBetaGlobWPRENeo
Plasmid#221898PurposeExpresses mStayGold targeted to the mitochondriaDepositorInsertcox8-mStayGold
UseTagsCox8ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-MitoTimer
Plasmid#50547PurposeExpresses the Timer protein targeted to the mitochondria under a Tet-inducible promoterDepositorInsertMitoTimer
UseTagsExpressionMammalianMutationF12S mutation in TimerPromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2 HRS-RFP
Plasmid#29685DepositorInserthepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1
Plasmid#41393Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1a.WPRE.SV40
Plasmid#100854PurposeAAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, and AAV9InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N174
Plasmid#66809Purpose2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistanceDepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRASV12
Plasmid#20205DepositorInsertNRas (NRAS Human)
UseTransposonTagsExpressionMutationG12VPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR (AAV1)
Viral Prep#112159-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSynap.iGABASnFR (#112159). In addition to the viral particles, you will also receive purified pAAV.hSynap.iGABASnFR plasmid DNA. Synapsin-driven iGABASnFR GABA sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-natMX6 (pFB1434)
Plasmid#126050PurposeFor C-terminal tagging with TurboID in yeast including nourseothricin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV Retrograde)
Viral Prep#87306-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre
Plasmid#13775PurposeMammalian expression of Cre recombinase from the broadly active CAG promoter/enhancerDepositorInsertCre
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorInsertDNMT1 (DNMT1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
UseTags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…TagsExpressionMutationP697S mutation in putative replicase. S239G and S…PromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only