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Plasmid#120573PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, Y40APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/E37A-IRES-Puro
Plasmid#120572PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, E37APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 AKT1
Plasmid#67102PurposeStandard for cell-free expression benchmarking.DepositorInsertAKT1 (AKT1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BMPR1A
Plasmid#67105PurposeStandard for cell-free expression benchmarking.DepositorInsertBMPR1A (BMPR1A Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EHD2
Plasmid#67107PurposeStandard for cell-free expression benchmarking.DepositorInsertEHD2 (EHD2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationS320T, E384D, T387A, H388Q, T405V, V407A, H418R, …PromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2H1
Plasmid#67108PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2H1 (GTF2H1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_D429A
Plasmid#51080Purposemammalian expression of ARAF D429ADepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationD429APromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK048
Plasmid#75230PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to Arg and cha…PromoterT7 and n/aAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01:01
Plasmid#203276PurposeOverexpress HLA alleleDepositorInsertA*11:01:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01
Plasmid#203281PurposeOverexpress HLA alleleDepositorInsertA*11:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPI-UPP1-3xFLAG
Plasmid#201644PurposeOver-expression of 3xFLAG-tagged human UPP1DepositorInsertUPP1 (UPP1 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102887PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-FAM208A
Plasmid#115845PurposeEncodes codon optimized HA tagged FAM208ADepositorInsertHA tagged FAM208A (C3orf63 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro
Plasmid#89991PurposeDonor template for generation of SOX2-ntdTomato reporter cell linesDepositorInsertSOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Gluc-RMA-IRES-EGFP
Plasmid#189629PurposeExpresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianMutationPromoterIRES and hSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts2
Plasmid#115872PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only