Addgene

We narrowed to 1,544 results for: nif

Showing: 1301 - 1320 of 1544 results

Results per page:

Sort results by:

pOPS0263

Plasmid

#133228

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPS0262

Plasmid

#133227

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AsCpf1-2NLS

Plasmid

#102565

Purpose

bacterial expression of AsCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-Stat3-t2A-mCherry

Plasmid

#127540

Purpose

Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders …

Promoter

CMV

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Sar1b-H79G-mOrange2

Plasmid

#166900

Purpose

mammalian expression of Sar1b-H79G tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sar1b

Use

Tags

mOrange2

Expression

Mammalian

Mutation

H79G

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAGFP-VSVG

Plasmid

#11915

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

(L64F/T65S/V163A/T203H). The PAGFP also contains …

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Sec23A

Plasmid

#166893

Purpose

mammalian expression of Sec23A tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG1

Plasmid

#111444

Purpose

Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVenus-VSVG

Plasmid

#11914

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLO 64.1

Plasmid

#123120

Purpose

E. coli expression vector for active deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Tags

Expression

Mutation

Promoter

NA

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Q71L

Promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-GalT

Plasmid

#11931

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Jennifer Tirnauer, Tim Mitchison

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLO2 casX

Plasmid

#123122

Purpose

E. coli expression vector for CasX sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge