Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1301 - 1320 of 1585 results

  2. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

    Enlarge

  3. pVC-FUS-R521C

    Plasmid
    #175067
    Purpose
    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    changed Arginin 521 to Cystein; mVenus: C-Termina…
    Promoter
    CMV Promoter
    Availability
    Academic Institutions and Nonprofits only
    175067-plasmid-map-sequence-id-362347

    Enlarge

  4. pORFE0010

    Plasmid
    #112074
    Purpose
    3xmVenus module CT
    Depositor
    Christian Chevalier
    Insert
    3xmVenus
    Use
    Tags
    Expression
    Plant
    Mutation
    Ser to Thr at amino acid 63 and Phe to Leu at pos…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112074-plasmid-map-sequence-id-217639

    Enlarge

  5. pEM12395

    Plasmid
    #213999
    Purpose
    PrpsM driven expression of mVenusNB
    Depositor
    Marc Erhardt
    Insert
    mVenusNB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrpsM
    Availability
    Academic Institutions and Nonprofits only
    213999-plasmid-map-sequence-id-424914

    Enlarge

  6. pcDNA3-RecQL4-mVen

    Plasmid
    #211403
    Purpose
    mammalian expression of human RecQL4 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211403-plasmid-map-sequence-id-419252

    Enlarge

  7. pcDNA3-TopBP1-mVen

    Plasmid
    #211415
    Purpose
    mammalian expression of human TopBP1 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211415-plasmid-map-sequence-id-419260

    Enlarge

  8. pcDNA3-RPA32-mVen

    Plasmid
    #211388
    Purpose
    mammalian expression of human RPA32 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211388-plasmid-map-sequence-id-420425

    Enlarge

  9. pCAG-EHC

    Plasmid
    #112619
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    112619-plasmid-map-sequence-id-226490

    Enlarge

  11. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

    Enlarge

  12. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168122-plasmid-map-sequence-id-332862

    Enlarge

  13. G15-CASE

    Plasmid
    #168126
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at E244/N245 within GNA15
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168126-plasmid-map-sequence-id-332871

    Enlarge

  14. pcDNA3-C16orf59-mVen

    Plasmid
    #211425
    Purpose
    mammalian expression of human C16orf59 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211425-plasmid-map-sequence-id-419247

    Enlarge

  15. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    PTEN exon 5 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

    Enlarge

  16. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

    Enlarge

  17. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 2 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68350-plasmid-map-sequence-id-119622

    Enlarge

  18. px330_P53 exon 7 gRNA

    Plasmid
    #68351
    Purpose
    px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 7 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68351-plasmid-map-sequence-id-119623

    Enlarge

  19. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

    Enlarge

  20. pcDNA3-Cdc45-mVen

    Plasmid
    #211390
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211390-plasmid-map-sequence-id-420427

    Enlarge

Showing: 1301 - 1320 of 1585 results