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Showing: 1301 - 1320 of 1640 results

  12. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pVG97

    Plasmid
    #164849
    Purpose
    Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    164849-plasmid-map-sequence-id-328012

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  16. pmVen-RPA70-IRES-RPA32-IRES-RPA14

    Plasmid
    #211431
    Purpose
    mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14
    Depositor
    Helmut Pospiech
    Insert
    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    Use
    Tags
    mVenus (on RPA70)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    211431-plasmid-map-sequence-id-420345

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Showing: 1301 - 1320 of 1640 results