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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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ePPEplus
Plasmid#205241PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112922PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_Mock
Plasmid#223565PurposeThe plasmid is expressed in mammalian cells as a control.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Dest
Plasmid#74281PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS1ClacZ
Plasmid#55171PurposelacZ-reporter vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIW447-CRISPR XYL2
Plasmid#98906PurposeK. marxianus CRISPR Plasmid for XYL2 editingDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_D2A_tdTomato
Plasmid#184045PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)DepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUDP004
Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-KRAB
Plasmid#126589PurposeExpresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backboneDepositorInsert2XMCP-KRAB
UseLentiviralTagsNLS-HAExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCW
Plasmid#62658PurposeMammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancerDepositorInsertPhiC31
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS pEGFP-N3 (1299)
Plasmid#62043Purposemammalian expression of NLS-EGFPDepositorInsertSV40 NLS
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS3Clux
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PTEF1-yEGFPCLN2PEST-pRS406
Plasmid#64406PurposeTEF promoter driven GFP expressionDepositorInsertTEF1 promoter (TEF1 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-Plus-mCherry
Plasmid#191231PurposeTo express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertAsCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationE174R, R951K, R955APromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only